§1Calculator

§2What this calculator assumes

That the labelled mass is delivered. It generally is not: peptide content, residual water and counter-ion each reduce the delivered peptide mass below the label, and none is visible from a purity figure. The size of that discrepancy can be worked through in the purity-against-content calculator.
That the diluent volume added is the volume measured. Reconstitution into a lyophilisate cake displaces a small volume, which this calculator does not model.
That the syringe is a U-100 insulin syringe, graduated in units of 0.01 mL.
That no peptide is lost to container or syringe surfaces. At concentrations below roughly 0.2 mg per millilitre and in the absence of a surfactant, adsorption removes a measurable fraction. The Institute cannot quantify that term without compound-specific data and does not attempt to.