Independent · non-commercial · publishes on a quarterly cycle|Current cycle 2026 Q3
Methodology|Interests|Funding|Corrections
Compound Evidence InstituteEvidence synthesis · established 2023Graded assessments of compounds, trials, methods and supply
Document set current to 30 July 2026
Defined term · Analytical chemistry

Reverse-phase HPLC

Separation on a hydrophobic stationary phase under an acidified aqueous–organic gradient, with ultraviolet detection at 214 nanometres. The Institute’s reference method for the chromatographic purity…

Document identifier
CEI-GT-129
Series
Defined term
Version
1.0
Published
30 Jul 2026
Last reviewed
30 Jul 2026
Certainty
Not rated

§1Reverse-phase HPLC

§1.1Also known as

RP-HPLC

§1.2Category

Analytical chemistry — Terms describing the determination of identity, purity, content and quality.

§1.3Related terms

  • Chromatographic purity — The area of the main peak as a percentage of total integrated area under stated conditions. A relative measure that assumes comparable molar absorptivity across species…
  • Gradient — The programmed change in mobile-phase composition over a chromatographic run. A shallow gradient resolves close-eluting species that a steep gradient co-elutes, and the…
Compound Evidence Institute · CEI-GT-129 · https://compoundevidence.com/glossary/reverse-phase-hplc/ · retrieved 30 July 2026
Nothing published by the Institute is medical advice, a diagnosis, a prescription, a treatment recommendation or a purchasing recommendation. Compounds supplied for research use are not approved for human or veterinary use in any jurisdiction, and a favourable analytical assessment of a supplier is not a statement that any product is safe or effective. The Institute publishes certainty ratings and never recommendations. No telephone number, messaging handle or ordering channel appears anywhere on this site.