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Compound Evidence InstituteEvidence synthesis · established 2023Graded assessments of compounds, trials, methods and supply
Document set current to 30 July 2026
Defined term · Analytical chemistry

Gradient

The programmed change in mobile-phase composition over a chromatographic run. A shallow gradient resolves close-eluting species that a steep gradient co-elutes, and the two report different purities…

Document identifier
CEI-GT-132
Series
Defined term
Version
1.0
Published
30 Jul 2026
Last reviewed
30 Jul 2026
Certainty
Not rated

§1Gradient

§1.2Category

Analytical chemistry — Terms describing the determination of identity, purity, content and quality.

§1.3Related terms

  • Reverse-phase HPLC — Separation on a hydrophobic stationary phase under an acidified aqueous–organic gradient, with ultraviolet detection at 214 nanometres. The Institute’s reference method…
Compound Evidence Institute · CEI-GT-132 · https://compoundevidence.com/glossary/gradient/ · retrieved 30 July 2026
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